Ss. Lee et al., INTRAVESICAL GENE-THERAPY - IN-VIVO GENE-TRANSFER USING RECOMBINANT VACCINIA VIRUS VECTORS, Cancer research, 54(13), 1994, pp. 3325-3328
Intratumoral gene transfer may be a significant tool in active immunot
herapy. The ability to insert functional genes into a tumor in vitro a
nd in vivo using recombinant vaccinia vectors was examined in the muri
ne bladder tumor model. Vaccinia recombinants expressing the influenza
hemagglutinin or nucleoprotein antigens infected/transfected murine (
MB-49 and MBT-2) and human (T24) bladder tumor cell lines in vitro. Sy
stemic vaccinia immunity was induced with as few as 10 plaque-forming
units of recombinant vaccinia instilled intravesically, and the encode
d protein was expressed in vivo in tumor and urothelium. However, prei
mmunity to vaccinia did not inhibit intravesical tumor transfection. T
hus, recombinant vaccinia virus is effective in introducing foreign an
tigens locally into tumor in vivo, supporting its use in clinical immu
notherapy.