Vacuolar type H+-ATPase (V-type ATPase) was isolated from a tonoplast
preparation of mature pear (Pyrus communis L. var. sativa DC, cv'La Fr
ance') fruit. This ATPase comprised 10 polypeptides of M(r) 68 000, 54
000 44 000, 42 000, 35 000, 30 000, 27 000, 16 000, 13 000 and 12 000
as subunits by SDS-PAGE. This ATPase was similar to the general V-typ
e ATPase.