A. Kolbe et al., PREGNANE-TYPE METABOLITES OF BRASSINOSTEROIDS IN CELL-SUSPENSION CULTURES OF ORNITHOPUS-SATIVUS, Phytochemistry, 36(3), 1994, pp. 671-673
Exogenously applied 24-epi-castasterone and 24-epi-brassinolide are tr
ansformed in a multi-step metabolic process by cell suspension culture
s of Ornithopus sativus to give 2 alpha,3 beta,6 beta-trihydroxy-5 alp
ha-pregnane-20-one and 2 alpha,3 beta-dihydroxy-B-homo-7-oxa-5 alpha-p
regnane-6,20-dione, respectively, as the major metabolites. This is th
e first report concerning a catabolic side-chain degradation of brassi
nosteroids in plant material.