PURIFICATION AND CHARACTERIZATION OF 2 FISH ENCEPHALITIS VIRUSES (FEV) INFECTING LATES-CALCARIFER AND DICENTRARCHUS-LABRAX

Authors
COMPS M PEPIN JF BONAMI JR
Citation
M. Comps et al., PURIFICATION AND CHARACTERIZATION OF 2 FISH ENCEPHALITIS VIRUSES (FEV) INFECTING LATES-CALCARIFER AND DICENTRARCHUS-LABRAX, Aquaculture, 123(1-2), 1994, pp. 1-10
Citations number
23
Categorie Soggetti
Fisheries,"Marine & Freshwater Biology
Journal title
Aquaculture → ACNP
ISSN journal
00448486
Volume
123
Issue
1-2
Year of publication
1994
Pages
1 - 10
Database
ISI
SICI code
0044-8486(1994)123:1-2<1:PACO2F>2.0.ZU;2-O
Abstract
Two viruses causing fish encephalitis (D1EV and LcEV) were isolated fr om larvae of Dicentrarchius labrax and Lates calcarifer collected from hatcheries in France and in Tahiti, respectively. In both cases, vira l particles were icosahedral, and measured 27-28 nm in negatively stai ned preparations. Complete particles exhibited a buoyant density of 1. 351. 36 g/ml in CsCl. The genome of each virus consisted of two pieces of ssRNA with molecular weights of 1.056 x 10(6) and 0.495 x 10(6) Da . Characterization of the virions' structural polypeptides on SDS-PAGE resolved two polypeptides with molecular weights of 42 and 40 kDa in D1EV or 42 and 36 kDa in LcEV. From their characteristics, these virus es are very similar and closely related to the members of the Nodaviri dae family. To distinguish them from the insect nodaviruses, we named provisionally these agents, fish encephalitis viruses (FEV).