SHOT-GUN SEQUENCING STRATEGY FOR LONG-RANGE GENOME MAPPING - A PILOT-STUDY

We have recently proposed a strategy for construction of long-range ph ysical maps based on random sequencing of NotI linking and jumping clo nes. Here, we present results of sequence comparison between 168 NotI linking (100 of them were sequenced from both sides) and 81 chromosome 3-specific jumping clones. We were able to identify 14 NotI jumping c lones (17%), each joined with two NotI linking clones. The average siz e of chromosomal jumps was about 650 kb. The assembled 42 NotI genomic fragments correspond to 12-15% of chromosome 3. These results demonst rate the value of random sequencing of NotI linking and jumping clones for genome mapping. This mapping proposal can be used for connecting physical and genetic maps of the human genome and will be a valuable s upplement to YAC and cosmid library based mapping projects. (C) 1994 A cademic Press, Inc.