Mc. Vidotto et al., IRON-REGULATED OUTER-MEMBRANE PROTEINS OF STRAINS OF AVIAN SEPTICEMICESCHERICHIA-COLI, Brazilian journal of medical and biological research, 27(6), 1994, pp. 1291-1297
1. Outer-membrane protein patterns of Escherichia coil recovered from
the peritoneal cavities of infected guinea pigs and grown in medium M9
containing 2,2'-dipyridyl were studied by polyacrylamide gel electrop
horesis (SDS-PAGE) to determine whether in vivo conditions of growth a
ffected the expression of these bacterial surface proteins. 2. Eleven
strains of septicemic E. coli studied in vitro under conditions of iro
n restriction expressed iron-regulated outer-membrane proteins, mainly
the protein of approximately 74 kDa, whereas avirulent strains grown
under similar conditions did not present the 74-kDa protein. 3. These
results show the distribution of iron-regulated outer-membrane protein
s among avian E. coli and suggest that the protein of approximately 74
kDa may be important for the virulence of these strains.