AVAILABILITY OF SYNTHETIC SALMON-CALCITONIN IN TISSUE-FLUID AFTER A SINGLE INTRAVENOUS DOSE

To obtain further information about the availability of salmon calcito nin in the biophase compartment that surrounds the receptor site, salm on calcitonin concentrations in plasma and skin blister fluid (SBF) af ter a single IV dose of 100 IU synthetic salmon calcitonin were compar ed in 15 healthy volunteers. Serial blood and SBF samples were collect ed before and up to 8h after administration and calcitonin was determi ned by a specific RIA. The maximum concentration in plasma was 225 pg. ml(-1) (in the first sample at 15 min), whereas in SBF the mean peak o f 84 pg.ml(-1) was reached after about 30 min. The distribution of sal mon calcitonin into SBF, defined as the ratio of the AUCs in SBF and p lasma, was 1.5. The kinetic profiles of salmon calcitonin in plasma an d interstitial fluid were different. Calcitonin in plasma peaked and t hen levelled out, while in SBF it persisted longer than in plasma. Thi s is the first report of the distribution of salmon calcitonin into bl ister fluid.