THE INFLUENCE OF GLYCATION ON THE PEROXIDASE-ACTIVITY OF HEMOGLOBIN

Authors
KHOO UY NEWMAN DJ MILLER WK PRICE CP
Citation
Uy. Khoo et al., THE INFLUENCE OF GLYCATION ON THE PEROXIDASE-ACTIVITY OF HEMOGLOBIN, European journal of clinical chemistry and clinical biochemistry, 32(6), 1994, pp. 435-440
Citations number
30
Categorie Soggetti
Biology,"Chemistry Medicinal
Journal title
European journal of clinical chemistry and clinical biochemistry → ACNP
ISSN journal
09394974
Volume
32
Issue
6
Year of publication
1994
Pages
435 - 440
Database
ISI
SICI code
0939-4974(1994)32:6<435:TIOGOT>2.0.ZU;2-J
Abstract
The peroxidase activity of haemoglobin A was characterized for non-gly cated and glycated haemoglobin (HbA1) within the pH range 4.5 to 6.0, by measuring the rate of oxidation of 5-aminosalicylic acid following the degradation of H2O2. Glycation was found to significantly lower th e pH activity of haemoglobin peroxidase throughout the pH range. Howev er, in the presence of 100 mmol/l sorbitol the pH activity profile of glycated haemoglobin was significantly elevated whilst that of non-gly cated haemoglobin remained unchanged.