MICROCHEMICAL STRUCTURAL DETERMINATION OF A PEPTOID COVALENTLY BOUND TO A POLYMERIC BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY
Ra. Zambias et al., MICROCHEMICAL STRUCTURAL DETERMINATION OF A PEPTOID COVALENTLY BOUND TO A POLYMERIC BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION TIME-OF-FLIGHT MASS-SPECTROMETRY, Tetrahedron letters, 35(25), 1994, pp. 4283-4286
New methodology utilizing matrix-assisted laser desorption ionization
time-of-flight mass spectroscopy (MALDI-TOF MS) for the direct identif
ication of a ligand covalently attached to a polymeric bead is reporte
d. The method allows for structure determination without relying on en
coding strategies. Gaseous TFA cleavage of a peptoid from a single bea
d followed by mounting in MALDI matrix and irradiation with a beam at
337 nm gave abundant molecular ions and structurally informative fragm
ent ions produced by post source decay (PSD).