SOLID-PHASE SYNTHESIS OF PEPTIDES USING ALLYLIC ANCHORING GROUPS .2. PALLADIUM-CATALYZED CLEAVAGE OF FMOC-PROTECTED PEPTIDES

High yields for the cleavage reaction of Fmoc-protected peptide segmen ts from an allylic handle may be obtained using tributyltin hydride in the presence of (Ph(3)P)PdCl2 in a 1:1 mixture of DMF/DCM. Alternativ ely the cleavage reaction may be carried out using NMA as nucleophile in a 2:2:1 mixture of DMSO/THF/0.5M HCl in the presence of (Ph(3)P)(4) Pd. The Fmoc group is completely stable to both these cleavage methods .