T. Ohsugi et al., ENGRAFTMENT OF HTLV-I-TRANSFORMED HUMAN T-CELL LINE INTO SCID MICE WITH NK CELL-FUNCTION, Journal of veterinary medical science, 56(3), 1994, pp. 601-603
HTLV-I-transformed human T-cell line. MT-2, has been reported to succe
ssfully proliferate in only the SCID mice with depletion of NK cell fu
nction. However, MT-2 cells could be engrafted into SCID mice possessi
ng normal NK cell function in this study. MT-2 cells (5-7 x 10(7) cell
s) were intraperitoneally injected into SCID mice without the deletion
of NK cell function. The SCID mice developed tumors in the peritoneal
cavities 3 months after the inoculation of MT-2 cells. All tumors rea
cted to anti-HTLV-I p19 and anti-HLA-DR monoclonal antibodies by immun
ofluorescence assay and were also positive for the HTLV-I gene by PCR
assay. DNA obtained from main organs in group of mice with or without
tumors showed a high incidence of positive signals for HTLV-I gene by
PCR assay.