EFFECTS OF PERFUSION RATE AND GLUCOSE-CONCENTRATION ON GLUCOSE-ABSORPTION IN INTESTINAL THIRY-VELLA LOOP IN SHEEP

The effects of the perfusion rate and glucose concentration in perfusa te on glucose absorption in the intestinal Thiry-Vella loop of conscio us sheep were studied. When 10 mM glucose was perfused at the rates of 0.5, 1, 2 and 4 ml/min. the absorption rates were 77.1+/-2.2, 54.9+/- 2.3, 31.6+/-2.1 and 12.9+/-0.3%, respectively. When 5, 10, 20 and 40 m M glucose solutions were perfused at 1 ml/min, the absorption rates we re 75.5+/-1.7, 52.2+/-2.2, 29.4+/-1.9 and 19.5+/-0.1%, respectively. W e conclude that the perfusion of 10 mM glucose solution at 1 ml/min, i n which about a 50% absorption rate was obtained. are the optimal cond itions for the study of glucose absorption in the intestinal loop of s heep.