IDENTIFICATION AND CHARACTERIZATION OF GENES INDUCED DURING SEXUAL-DIFFERENTIATION IN SCHIZOSACCHAROMYCES-POMBE

Authors
SATO S SUZUKI H WIDYASTUTI U HOTTA Y TABATA S
Citation
S. Sato et al., IDENTIFICATION AND CHARACTERIZATION OF GENES INDUCED DURING SEXUAL-DIFFERENTIATION IN SCHIZOSACCHAROMYCES-POMBE, Current genetics, 26(1), 1994, pp. 31-37
Citations number
36
Categorie Soggetti
Genetics & Heredity
Journal title
Current genetics → ACNP
ISSN journal
01728083
Volume
26
Issue
1
Year of publication
1994
Pages
31 - 37
Database
ISI
SICI code
0172-8083(1994)26:1<31:IACOGI>2.0.ZU;2-W
Abstract
Five cDNA clones, harboring genetic messages preferentially expressed during the sexual differentiation process, were isolated from a cDNA l ibrary of Schizosaccharomyces pombe by subtractive screening. Transcri ption of the corresponding genes, termed isp3, 4, 5, 6, and 7, was dep endent on nitrogen starvation and their induction occurred at several stages of spore formation. Analysis of the cDNA primary structures rev ealed a capacity for the coding of polypeptides of 19.2 kDa, 88.3 kDa, 60.1 kDa, 49.7 kDa, and 43.8 kDa, respectively. The translated amino- acid sequences of isp5 and isp6 were found to show significant similar ities to those of amino-acid permeases and proteinase B of Saccharomyc es cerevisiae, respectively. Disruption of isp6 arrested the cell cycl e prior to conjugation and caused a drastic blocking effect on spore f ormation.