PROBE PH2-42 OF HUMAN LOCUS D13S25 - SEQUENCE, LOCALIZATION, AND POLYMERASE CHAIN-REACTION MARKERS

Authors
SULIMOVA GE ZAKHAREV VM UDINA IG BLISKOVSKII VV KIRILLOV AV SHCHEPINOV MS VOROBEVA NV KAPANADZE BI GRAFODATSKII AS YANKOVSKII NK
Citation
Ge. Sulimova et al., PROBE PH2-42 OF HUMAN LOCUS D13S25 - SEQUENCE, LOCALIZATION, AND POLYMERASE CHAIN-REACTION MARKERS, Molecular biology, 27(4), 1993, pp. 560-564
Citations number
11
Categorie Soggetti
Biology
Journal title
Molecular biology → ACNP
ISSN journal
00268933
Volume
27
Issue
4
Year of publication
1993
Part
2
Pages
560 - 564
Database
ISI
SICI code
0026-8933(1993)27:4<560:PPOHLD>2.0.ZU;2-C
Abstract
The HindII-HindII fragment of probe pH2-42 of human genome locus D13S2 5 was sequenced, and its localization in the q14-q21 region of chromos ome 13 confirmed by in situ hybridization. Seven oligonucleotide prime rs for PCR were selected to amplify fragments almost completely coveri ng the pH2-42 sequence. The data of Bowcock and Hebert [Nucl. Acids Re s., 18, 7194 (1990)] and of the present work were used to reconstruct the distribution of polymorphic SspI sites in D13S25. This makes the p rimer set applicable not only for screening cosmid and YAC libraries b ut also for marking the given locus of human chromosome 13.