2-SITE IMMUNORADIOMETRIC ASSAY (IRMA) - DETECTION, EFFICIENCY, AND PROCEDURAL MODIFICATIONS

Authors
NANDA N JOSHI H SUBBARAO SK SHARMA VP
Citation
N. Nanda et al., 2-SITE IMMUNORADIOMETRIC ASSAY (IRMA) - DETECTION, EFFICIENCY, AND PROCEDURAL MODIFICATIONS, Journal of the American Mosquito Control Association, 10(2), 1994, pp. 225-227
Citations number
8
Categorie Soggetti
Entomology
Journal title
Journal of the American Mosquito Control Association → ACNP
ISSN journal
8756971X
Volume
10
Issue
2
Year of publication
1994
Part
1
Pages
225 - 227
Database
ISI
SICI code
8756-971X(1994)10:2<225:2IA(-D>2.0.ZU;2-Y
Abstract
A 2-site immunoradiometric assay (IRMA) has been done using Anopheles stephensi fed on Plasmodium vivax blood through parafilm to ascertain at what time in the sporogonic cycle circumsporozoite (CS) antigen can be detected, and to detect CS antigen in mosquitoes squashed on filte r paper (FP) and cellulose acetate membrane (CAM). The CS antigen was detectable only in mature oocysts, a day prior to sporozoite liberatio n, and in salivary gland sporozoites. Dissected sporozoites adsorbed o n FP/CAM also can be analyzed by IRMA for Plasmodium species identific ation.