EMERGENCE OF INFLAMMATORY ALVEOLAR MACROPHAGES DURING REJECTION OR INFECTION AFTER LUNG TRANSPLANTATION

Citation
I. Frachon et al., EMERGENCE OF INFLAMMATORY ALVEOLAR MACROPHAGES DURING REJECTION OR INFECTION AFTER LUNG TRANSPLANTATION, Transplantation, 57(11), 1994, pp. 1621-1628
Citations number
27
Categorie Soggetti
Immunology,Surgery
Journal title
ISSN journal
00411337
Volume
57
Issue
11
Year of publication
1994
Pages
1621 - 1628
Database
ISI
SICI code
0041-1337(1994)57:11<1621:EOIAMD>2.0.ZU;2-7
Abstract
Local activation of macrophages may play an important role in immune c omplications following lung transplantation. To document such a phenom enon, we have investigated the possible changes of alveolar macrophage surface antigen expression after lung transplantation. Using immunocy tofluorometry, we have analyzed the phenotype of alveolar macrophages from 41 bronchoalveolar lavage fluids obtained from 19 lung transplant recipients displaying various complications. The strong expression of HLA-DR observed on almost all alveolar macrophages was similar among groups I (no complication), II (minimal acute rejection), and III (mil d to severe acute rejection), but was enhanced in group IV (bronchial infection) (P<0.03). We observed no significant variation in the monoc yte lineage CD14 antigen expression among the 4 groups, and about 83% of alveolar macrophages expressed this marker strongly. Membrane expre ssion of the 27E10 antigen that characterizes infiltrating macrophages in acute inflammatory lesions was significantly higher during mild to severe rejection episodes than in controls (P<0.02) and during bronch ial infections (P<0.05) but not during minimal rejection. Double stain ing experiments confirmed that 27E10-positive cells in groups III and IV belonged to the macrophage lineage. In addition, the expression of the 27E10 antigen on cultured alveolar macrophages was found to be inc reased after stimulation by bacterial lipopolysaccharide or IFN-gamma. These results indicate that a particular alveolar macrophage subpopul ation is activated during immune events after lung transplantation. Th is population, recognized by the 27E10 mAb, might be involved in cytok ine production during severe acute rejection and infection episodes.