DEVELOPMENT OF A NEEDLE TYPE GLUCOSE BIOSENSOR

A needle type glucose biosensor has been developed for monitoring of g lucose levels during hemorrhagic shock. The sensor employs polycarbona te membranes that are either untreated or treated with Nafion or Silas tic, and Glucose Oxidase enzyme which is immobilized on modified carbo n powder in a gel matrix. Sensor performance was evaluated in vitro an d the sensor showed a sensitivity of up to 10 nA/mM and a linear range of up to 30 mM. Evaluation of the sensor response in serum showed sim ilar sensitivity and linear range as obtained using calibration curves in buffer solution. The sensors were operated continuously for 28 day s in phosphate buffer solution and no significant change in the sensit ivity and the linear range was observed during the first 7 days. Senso rs show a minimum change in their performance when stored inactive in buffer at 4 degrees C for at least eight weeks.