A PIEZOELECTRIC QUARTZ-CRYSTAL BIOSENSOR AS A DIRECT AFFINITY SENSOR

Authors
MINUNNI M SKLADAL P MASCINI M
Citation
M. Minunni et al., A PIEZOELECTRIC QUARTZ-CRYSTAL BIOSENSOR AS A DIRECT AFFINITY SENSOR, Analytical letters, 27(8), 1994, pp. 1475-1487
Citations number
22
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical letters → ACNP
ISSN journal
00032719
Volume
27
Issue
8
Year of publication
1994
Pages
1475 - 1487
Database
ISI
SICI code
0003-2719(1994)27:8<1475:APQBAA>2.0.ZU;2-Z
Abstract
A microprocessor controlled piezoelectric detector as sensor was emplo yed to monitor in real time protein adsorption and immunoreactions usi ng piezoelectric quartz crystals (AT-cut) with a basic resonant freque ncy of 10 MHz. The adsorbed protein was an immunoglobulin (h-lg G); in the immunosensing a covalent immobilized molecule (the pesticide 2,4- D) formed the receptor for the immobilized ligand sample (Mab anti-2,4 -D) in a competitive assay.