SPECTROPHOTOMETRIC AND INDIRECT DETERMINATION OF LINCOMYCIN BY ATOMIC-ABSORPTION SPECTROSCOPY (AAS)

The reaction of lincomycin with cupric ions in alkaline medium was tak en as a basis for the colorimetric and indirect atomic absorption spec trometric ( AAS ) determination of lincomycin hydrochloride. The AAS p rocedure was based on the extraction of copper-lincomycin complex at p H 11, into n-butanol. The copper content in this extract was determine d by AAS. The response was linear for up to 30 mu g ml(-1) of lincomyc in. The method is accurate sensitive and simple. The proposed methods were applied to pharmaceutical preparations with fine accuracy.