CYTOPHOTOMETRIC DETERMINATION OF DNA CONT ENT IN NUCLEAR SUSPENSIONS CONSERVED FOR PROLONGED PERIODS - PRELIMINARY-STUDY

The variations in cytofotometric determination of DNA content were stu died in a concentrated suspension of nuclei stored for prolonged perio ds. These came from normal and tumoral cells of an uterine leiomyosarc oma, fixed in formalin and included in paraffine. DNA was measured in normal and tumoral cells at days 0, 1, 7, 11, 19, 27 and 33. No signif icant variations in DNA measurement were observed. In a same day, ther e was an uniform DNA content, ploidy pattern and DNA index and an aneu ploid pattern was diagnosed in all histograms. These results suggest t hat nuclear suspensions can be conserved for long periods and used for DNA content determination, allowing the access of remote places to th ese techniques.