REFINING THE DESCRIPTION OF PROTEIN CHROMATOGRAPHY

Review of design procedures used for protein chromatography shows the need for considerable refinement to meet the demands of high flow-rate s and complex processes characteristic of modern commercial operation. Suggestions are made for dealing with the skewness of effluent curves from differential chromatography in interpreting such data and also f or scaling up laboratory processes. Proton-specific nuclear magnetic r esonance images of small commercial columns show significant non-unifo rmity of packing density, and suggestions are made for a study of the effects of column shape, size and packing procedures on column efficie ncy.