DIAGNOSIS OF HIV-I INFECTION BY DETECTION OF ANTIBODY IGG TO HIV-1 INURINE WITH ULTRASENSITIVE ENZYME-IMMUNOASSAY (IMMUNE-COMPLEX TRANSFERENZYME-IMMUNOASSAY) USING RECOMBINANT PROTEINS AS ANTIGENS

Anti-HIV-1 IgG in urine was detected by an ultrasensitive enzyme immun oassay (immune complex transfer enzyme immunoassay) using recombinant reverse transcriptase (RT), p17 and p24 as antigens, and beta-D-galact osidase from Escherichia coli as label. Anti-HIV-1 IgG in urine was re acted simultaneously with 2,4-dinitrophenyl-bovine serum albumin-recom binant protein conjugate and recombinant protein-beta-D-galactosidase conjugate. The immune complex formed, consisting of the three componen ts, was trapped onto polystyrene balls coated with affinity-purified ( anti-2,4-dinitrophenyl group) IgG. After washing, the immune complex w as eluted from the polystyrene balls with excess of epsilon N-2,4-dini trophenyl-L-lysine and transferred to clean polystyrene balls coated w ith