EFFECTS OF MEMBRANE DEPOLARIZATION AND CHANGES IN INTRACELLULAR AND EXTRACELLULAR CALCIUM-CONCENTRATION ON PHOSPHOINOSITIDE HYDROLYSIS IN BOVINE TRACHEAL SMOOTH-MUSCLE
Er. Chilvers et al., EFFECTS OF MEMBRANE DEPOLARIZATION AND CHANGES IN INTRACELLULAR AND EXTRACELLULAR CALCIUM-CONCENTRATION ON PHOSPHOINOSITIDE HYDROLYSIS IN BOVINE TRACHEAL SMOOTH-MUSCLE, Biochemical pharmacology, 47(12), 1994, pp. 2171-2179
Agonist-stimulatd phosphoinositide metabolism plays a central role in
pharmacomechanical coupling in airways smooth muscle (ASM). In many ot
her tissues and cells, most noteably excitable cells, membrane depolar
ization or an increase in intracellular Ca2+ ([Ca2+](i)) generated by
inositol 1,4,5-trisphosphate (Ins(1,4,5)P-3)-induced Ca2+ release or a
gonist-mediated Ca2+ influx is able to trigger or augment phosphaticdy
linositol 4,5-bisphosphate (PtdIns(4,5)P-2) hydrolysis and/or initiate
PtdIns4P/PtdIns hydrolysis by direct stimulation of PIC. To assess th
e importance of these mechanisms in ASM the effects of KCl-induced mem
brane depolarization, extracellular Ca2+ ([Ca2+](e)) chelation, and ad
dition of ionomycin to elevate [Ca2+](i) on basal and agonist-stimulat
ed Ins(1,4,5)P-3 concentration and [H-3]-InsP(x) accumulation have bee
n examined. Reducing [Ca2+](e) from 1.8 mM to 6 or 0.8 mu M caused a p
rogressive inhibition of agonistic-stimulated [H-3]inositol polyphosph
ate accumulation over 30 min with the histamine-stimulated response be
ing significantly more sensitive to [Ca2+](e) chelation than the respo
nse to carbachol. In contrast, the initial accumulation of Ins(1,4,5)P
-3 was completely unaffected by such reductions in [Ca2+](e). Incubati
on of [H-3]inositol-prelabelled BTSM slices with buffer containing 80
mM KCl failed to stimulat [H-3]InsP(x) accumulation, causing instead a
small inhibition of carbachol-stimulated [H-3]InsP(x) accumulation wi
th a similar effect seen with respect to Ins(1,4,5)P-3 accumulation. A
ddition of 5 mu M ionomycin to BTSM slices similarily did not stimulat
e Ins(1,4,5)P-3 generation and only increased [H-3]InsP(x) accumulatio
n after prolonged stimulation in the presence of high (mM) [Ca2+](e).
These data indicated that in ASM, membrane depolarization or physiolog
ical increases in [Ca2+](i) did not result in either independent activ
ation of PIC or augmentation of initial agonist-stimulated PtdIns(4,5)
P-2 hydrolysis. However, while the initial agonist-stimulated generati
on of Ins(1,4,5)P-3 was not dependent on [Ca2+](e), a normal plasmalem
mal Ca2+ gradient was required to sustain maximal rates of agonist-sti
mulated PtdIns(4,5)P-2 hydrolysis.