MICROWAVE-ANTIGEN RETRIEVAL FOR PROLIFERATION ANALYSIS (MIB-1) AND QUANTICYT KARYOMETRY OF BLADDER WASHINGS

Bladder washings can be used for simultaneous karyometry and prolifera tion analysis using MiB-1 as a proliferation marker. We analyzed 42 pr oblem cases with a discordance between cytologic and karyometric class ification (QUANTICYT), in which the karyometric classification was bas ed on a combination of a nuclear shape parameter and DNA (2cDI). Moreo ver, 25 concordant cases were analyzed: 5 normal samples, 6 low-grade tumors and 7 high-grade tumors. All normal samples and all low-grade t umors had labelling indices below 10%, and all high-grade tumors over 10%. For the discordant low-grade tumors, the QUANTICYT classification correlated better with the MiB-1 labelling than the cytologic diagnos is. There was a clear correlation between 2cDI and MiB-1 labelling ind ex. Slightly elevated MiB-1 labelling indices might have some prognost ic value.