DIFFERENTIAL ROLES OF AT(1) AND AT(2) RECEPTOR SUBTYPES IN VASCULAR TROPHIC AND PHENOTYPIC CHANGES IN RESPONSE TO STIMULATION WITH ANGIOTENSIN-II

The aim of this study was to investigate the roles of angiotensin II ( Ang II) receptor subtypes 1 (AT(1)) and 2 (AT(2)) in producing vascula r wall hypertrophy and qualitative changes in smooth muscle cell gene expression. Wistar rats were treated for 23 days with osmotic minipump s containing solvent and either Ang II (120 ng . kg(-1). min(-1)) or P D123319 (30 mg . kg(-1). d(-1)), an AT(2) receptor antagonist. In addi tion, rats receiving solvent and either Ang II or PD123319 were given losartan, an AT(1) receptor antagonist, in the drinking water (10 mg . kg(-1). d(-1)). Vascular wall hypertrophy and smooth muscle phenotype were characterized by morphometric analysis combined with immunohisto chemistry. Ang II-induced hypertension was associated with the develop ment of medial hypertrophy of the aorta and coronary arteries accompan ied by reversion of vascular smooth muscle cells (VSMCs) toward an imm ature phenotype, as shown by the expression of cellular fibronectin an d nonmuscle myosin. Losartan treatment, which restored normal arterial pressure, prevented all these changes. PD123319 treatment, which had no effect on blood pressure, prevented only vascular hypertrophy, with no effect on VSMC phenotype. Administration of only losartan to norma l rats reproduced the Ang II-induced vascular hypertrophy, with no eff ect on VSMC phenotype. Taken together, these results suggest that (1) the trophic effect of Ang II on VSMCs is mediated via AT(2) receptor s ubtypes and (2) changes in VSMC phenotypes are triggered mainly throug h AT(1) receptor subtypes.