LIGHT-MICROSCOPIC LECTIN HISTOCHEMISTRY IN AGING MOUSE KIDNEY - STUDYOF COMPOSITIONAL CHANGES IN GLYCOCONJUGATES

We report compositional changes in glyconjugates in mouse kidney corte x due to aging, as analyzed by lectin histochemistry and Western blot analysis. Mouse kidney tissues of prenatal and postnatal ages (prenata l, 19 days of gestation; postnatal 2 and 8 days, 4 and 13 weeks, and 1 0 months) were fixed in 4% paraformaldehyde and cryosections were made . They were stained with 16 kinds of biotinylated lectin, followed by ABC, for light microscopy. Tissue homogenate was also examined by West ern blotting for WGA, ConA, and Lotus. Changes in glycoconjugates due to prenatal and postnatal aging were detected by both lectin histochem istry and Western blotting.