Three hundred and ninety-five primary root caries lesions in 117 patie
nts were included in this study. Each lesion was clinically characteri
sed (colour dimensions, distance from gingival margin and texture) and
a sample of infected or altered dentine was taken using a standardise
d sampling procedure. The relationships between the colour and the mic
roflora, texture, physical dimensions and distance from the gingival m
argin of the lesions were investigated. Lesions in each colour group (
light brown, yellow, dark brown and black) were identified in each tex
ture category (soft, leathery and hard), except for black lesions whic
h were recorded as only soft or leathery. Black soft and black leather
y lesions had a significantly greater area and harboured more lactobac
illi than all other types of lesions while black leathery lesions also
harboured significantly more yeasts. There were no significant differ
ences in the number of bacteria or mutans streptococci isolated from s
oft or leathery lesions irrespective of lesion colour. Overall, these
data indicate that the colour of primary root caries lesions is not a
reliable indicator of primary root caries activity. Assessment of the
clinical severity of primary root caries lesions requires consideratio
n of lesion texture and the distance of the lesion from the gingival m
argin rather than the colour of the lesion.