PLANT-REGENERATION FROM CALLUS-CULTURES OF ALFALFA

The morphogenic calluses and plant regeneration of alfalfa cv. Lucia f rom leaves, petiole and stem segments were induced. For callus inducti on a cultivation medium GMK with added growth regulators 2,4-D (8 mg.1 -1), KIN (8 mg.1-1) and NAA (0.5 mg.1-1) were used. The morphogenic pr ocesses in calluses were induced on B5 medium with BAP (0.2 mg.1-1) fo llowed on GMR medium (without growth regulators). Green globular struc tures arising from the calluses formed either shoots and roots simulta neously or shoots only. Roots were induced on rooting medium GMZ (half strength GM medium). Obtained rooted alfalfa regenerants were transfe rred into the soil and gradually adapted to the artificial cultivation conditions.