AMINE OXIDASE IN DEVELOPING POPPY SEEDLINGS, PAPAVER-SOMNIFERUM L

Amine oxidase activities from pea (Pisum sativum L.) and poppy (Papave r somniferum L.) seedlings were tested by using aromatic amines as sub strates (benzylamine, tyramine, dopamine, phenylethylamine and tryptam ine). The changes in UV-spectra during enzyme reactions tested were co mpared for individual substrates. Identical changes in UV-spectra for dopamine by using of both enzyme preparations and similarity of amine oxidase bands on nondenaturing PAGE indicate a dopamine specific amine oxidase in poppy seedlings. Results are discussed in relation to the biosynthesis of reticuline.