1. Acuity cards are now commonly used to assess the grating acuity of
infants, but the procedures vary across clinical settings. To evaluate
differences between various acuity card procedures and a more objecti
ve preferential looking test of acuity, we compared each of three acui
ty card procedures with a preferential looking (PL) staircase procedur
e in toddlers with ocular disorders. 2. In Expt 1, we used PL and two
or three variations of the acuity card procedure to measure grating ac
uity in one eye of 30 toddlers aged 15-30 months expected to have redu
ced acuity because of structural ocular abnormalities. For the acuity
card procedures, observers knew either the stripes' location (random p
rocedure), their relative size (Dobson procedure), or their location a
nd absolute size (informed procedure). For comparison, we tested 38 ad
ditional children, aged 16-32 months, with two tests of PL. 3. All thr
ee acuity card procedures yielded results which agreed moderately well
with PL, with Dobson's procedure agreeing best overall. However, the
acuity card procedures agreed with PL within 1/2 octave only 44-67% of
the time, whereas two tests of PL agreed within 1/2 octave 79% of the
time. A comparison of the results from the different acuity card proc
edures did not correspond to those that would be predicted by observer
bias. 4. In Expt 2, we tested interocular acuity differences using PL
and the Dobson acuity card procedure in 30 children aged 4-35 months
who were suspected to be amblyopic. 5. PL and acuity cards agreed for
only half the patients on whether or not there was an interocular diff
erence of at least 1 octave. A comparison of the results from each pro
cedure to the expected interocular acuity differences based on medical
history indicated that the acuity card procedure was either (a) influ
enced by an observer's expectation of interocular differences or (b) m
ore sensitive for detecting expected interocular differences than is P
L. 6. We conclude that acuity cards, used with these procedures. canno
t substitute for PL, but can yield moderately similar results in much
less time.