C. Sevcik et al., SPECIFIC BLOCKAGE OF SQUID AXON RESTING POTASSIUM PERMEABILITY BY HALICLONA-VIRIDIS (PORIFERA, HALICLONIDAE) TOXIN (HVTX), Toxicon, 32(7), 1994, pp. 773-788
The action of partially purified HvTX, toxin of the marine sponge H. v
iridis, was explored on the giant axon of the tropical squids Doryteut
his plei and Sepioteuthis sepioidea. HvTX depolarizes the nerves dose
dependently. The effect occurs after blocking sodium channels with tet
rodotoxin (1 mu M), removing external Na+, blocking electrically excit
able K+ channels with 3,4-diamino-pyridine (10 mM) or internal and ext
ernal application of tetraethylammonium (40 mM). Ouabain (up to 10 mM)
does not modify HvTX effect. The action of HvTX occurs only when it i
s applied to the outer phase of the nerve membrane; microinjection of
the toxin into the axons lacks depolarizing effects. HvTX reduces the
dependence of membrane potential on external potassium concentration.
The apparent Rb-86(+) permeability (pi') was measured in axons of S. s
epioidea. The value of pi' in normal artificial sea water was 80 (61,
96) nm/sec (median and its 95% confidence interval, n = 8) and raised
to 1030 (588, 2113) nm/sec (n = 7) when the axons were depolarized to
0 mV raising external K+ to 300 mM. In axons depolarized with HvTX (10
mM external K+) to 0 mV, pi' was 88 (55, 97) nm/sec (n = 8). HvTX cou
ld not prevent (P>>0.05) the increase in pi' induced by 300 mM K+ when
the ion concentration was raised before toxin application [pi' = 660
(354, 1876) nm/sec, n = 7]. Most of the Rb-86(+) permeability increase
in high K+ was prevented if HvTX was added before external K+ was rai
sed [pi' = 298 (264, 337) nm/sec, n = 8]. All the measures of pi' were
carried out in solutions containing 1 mu M tetrodotoxin, 1 mM 3,4-dia
minopyridine and 2 mM ouabain.