RAPID METHOD FOR ISOLATING DETERGENT-INSOLUBLE OUTER-MEMBRANE PROTEINS FROM PSEUDOMONAS-AERUGINOSA

The present study compares two techniques for isolating outer membrane proteins (OMPs) of Pseudomonas aeruginosa, Method A - selective solub ilization with sodium lauryl sarcosinate, and Method B - isopycnic suc rose gradient centrifugation, using three criteria: the amount of prot eins obtained, polypeptide patterns after sodium dodecyl sulfate-polya crylamide gel electrophoresis, and their practical aspects. Method A a ppears to be superior to Method B. It yields a higher outer membrane p rotein content and a similar polypeptide pattern as Method B, but is m ore rapid and less cumbersome.