STUDY OF MACROBIOMOLECULE-LIGAND INTERACTIONS BY LIQUID-CHROMATOGRAPHIC SEPARATION METHODS UNDER EQUILIBRIUM AND NONEQUILIBRIUM CONDITIONS

The interaction of propranolol with pure human al glycoprotein as well as that of bilirubin with complete human serum was studied using high -performance liquid chromatography. Hummel and Dreyer (equilibrium) si ze exclusion as well as (nonequilibrium) zonal elution chromatographic methods were applied. The binding interaction data were evaluated acc ording to three different approaches introduced by Scatchard, Bjerrum, and by Tobler and Engel. Procedure schedules designed for the study a nd evaluation of the reversible (equilibrium) macrobiomolecule-ligand interaction are discussed and critically commented.