Rl. Mendes et al., SUPERCRITICAL CARBON-DIOXIDE EXTRACTION OF HYDROCARBONS FROM THE MICROALGA BOTRYOCOCCUS-BRAUNII, Journal of applied phycology, 6(3), 1994, pp. 289-293
Samples of the microalga Botryococcus braunii were submitted to superc
ritical fluid extraction with carbon dioxide at 40-degrees-C and press
ures of 12.5, 20.0 and 30.0 MPa. The extraction yield and the fraction
of the hydrocarbons in the extracts both increased with pressure and
at 30 MPa these compounds were obtained rapidly. This behaviour is ass
ociated with the localization of the hydrocarbons outside the cell wal
l. In the extracts, which are fluid, golden and limpid, chlorophyll an
d phospholipids were not detected.