REMOVAL AND INACTIVATION OF ENVELOPED AND NONENVELOPED VIRUSES DURINGTHE PURIFICATION OF A HIGH-PURITY FACTOR-IX BY METAL CHELATE AFFINITY-CHROMATOGRAPHY

Authors
ROBERTS PL WALKER CP FELDMAN PA
Citation
Pl. Roberts et al., REMOVAL AND INACTIVATION OF ENVELOPED AND NONENVELOPED VIRUSES DURINGTHE PURIFICATION OF A HIGH-PURITY FACTOR-IX BY METAL CHELATE AFFINITY-CHROMATOGRAPHY, Vox sanguinis, 67, 1994, pp. 69-71
Citations number
8
Categorie Soggetti
Hematology
Journal title
Vox sanguinis → ACNP
ISSN journal
00429007
Volume
67
Year of publication
1994
Supplement
1
Pages
69 - 71
Database
ISI
SICI code
0042-9007(1994)67:<69:RAIOEA>2.0.ZU;2-N
Abstract
Virus reduction during the copper chelate affinity chromatography stag e used during the purification of a new high-purity factor IX (BPLs 9M C) has been investigated. Virus reduction for the enveloped virus Sind bis was 6.5 log, a value which included approximately 2 log of inactiv ation due to the use of an acidic wash buffer (pH 4.4) during chromato graphy. In the case of the non-enveloped hepatitis-A-like poliovirus, which is acid-resistant, the virus reduction value was 4.0 log and was exclusively due to physical virus removal during the chromatographic process.