NUCLEOTIDE-SEQUENCE ANALYSIS OF AN 11.7 KB FRAGMENT OF YEAST CHROMOSOME-II INCLUDING BEM1, A NEW GENE OF THE WD-40 REPEAT FAMILY AND A NEW MEMBER OF THE KRE2 MNT1 FAMILY/

This paper reports the DNA sequence and analysis of an 11.7kb segment localized on the right arm of Saccharomyces cerevisiae chromosome II. This fragment contains one incomplete and five long and non-overlappin g open reading frames (ORFs) designated from centromere to telomere-pr oximal side as: YBR1406, 1409, 1410, 1411, 1412 and 1413. YBR1406 corr esponds to the 5' end to PGI1 encoding phosphoglucoisomerase. YBR1410 encodes a polypeptide of 798 amino acids whose C terminus contains fiv e repeats (WD-40 repeat) similar to those found in the beta-subunits o f G proteins and different yeast proteins such as Tup1, Prp4 and Cdc4. The higher similarity score is obtained with dTAF(II)80, a component of the RNA polymerase II transcriptional complex TFIID. YBR1411 encode s a polypeptide of 464 amino acids which belongs to the family of alph a-mannosyltransferases: KRE2/MNT1, KTR1, KTR2, YUR1 and the product of previously sequenced ORF YBR1445. YBR1412 corresponds to BEM1. The tw o ORFs, YBR1409 and YBR1413, which do not exhibit significant similari ty with any known coding sequences, define new genes. The sequence has been deposited in the EMBL Data Library under Accession Number Z21487 .