P. Innocentifrancillard et al., BLOOD MONOCYTES INFECTED IN-VIVO BY HIV-1 VARIANTS WITH A SYNCYTIUM-INDUCING PHENOTYPE, AIDS research and human retroviruses, 10(6), 1994, pp. 683-690
Extensive data have been obtained on sequence changes in the V3 region
of the HIV-1 envelope protein that are associated with in vitro biolo
gical properties such as cell tropism and syncytium-inducing capacity.
However, so far this concerned viruses isolated from peripheral blood
mononuclear cells and thus did not discriminate between variants pres
ent in T lymphocytes or in monocytes. In this study, we analyzed viral
sequences derived separately from uncultured T lymphocytes, blood mon
ocytes, and plasma of an HIV-1-infected patient showing a neurological
evolution of the disease. Sequences related to the V3 region and 18 a
mino acids downstream were obtained from 48 clones after PCR amplifica
tion. One predominant viral sequence close to the monocytotropic/non-s
yncytium-inducing (NSI) consensus sequence was observed in the three b
lood sources. Two viral species were specifically identified in monocy
tes (43% of the clones), showing clear differences from the consensus
sequence and exhibiting the genetic determinants associated with the S
I phenotype. Plasma-derived viruses with a similar V3 loop were obtain
ed on in vitro isolation. Analysis of the biological properties of the
se selected viruses confirmed their monocytotropism and the syncytium-
inducing phenotype as expected by the cell type in which the sequences
were observed and the charge of the V3 loop. Structural analysis of t
hese variants suggested an intermediate structure between NSI/monocyto
tropic and SL/lymphotropic V3 loops. Thus, in vivo circulating monocyt
es could be a reservoir for distinct HIV-1 variants with potential SI
characteristics, at least in later stages of infection. Studying such
variants over the course of the infection may shed light on their invo
lvement in disease manifestations.