SIMILAR TOXIC EFFECT OF 1,3-BIS(2-CHLOROETHYL)-1-NITROSOUREA ON LYMPHOCYTES FROM HUMAN-SUBJECTS DIFFERING IN THE EXPRESSION OF GLUTATHIONE TRANSFERASE M1-1

Authors
JUNGNELIUS U RIDDERSTROM M HANSSON J RINGBORG U MANNERVIK B
Citation
U. Jungnelius et al., SIMILAR TOXIC EFFECT OF 1,3-BIS(2-CHLOROETHYL)-1-NITROSOUREA ON LYMPHOCYTES FROM HUMAN-SUBJECTS DIFFERING IN THE EXPRESSION OF GLUTATHIONE TRANSFERASE M1-1, Biochemical pharmacology, 47(10), 1994, pp. 1777-1780
Citations number
40
Categorie Soggetti
Pharmacology & Pharmacy",Biology
Journal title
Biochemical pharmacology → ACNP
ISSN journal
00062952
Volume
47
Issue
10
Year of publication
1994
Pages
1777 - 1780
Database
ISI
SICI code
0006-2952(1994)47:10<1777:STEO1O>2.0.ZU;2-2
Abstract
Sixteen healthy donors were investigated for the presence or absence o f glutathione transferase (GST) M1-1 in lymphocytes by immunodetection with polyclonal antibodies against human GST M1-1. Nine out of 16 ind ividuals (56%) were categorized as GST M1-1 positive. Phytohaemaggluti nin stimulated lymphocytes from GST M1-1 positive and negative donors were treated with 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) and comp ared regarding inhibition of [H-3]thymidine incorporation as a measure of cytotoxicity. No significant differences in the effect of BCNU wer e observed between the two groups, indicating that GST M1-1 is not an important resistance factor for BCNU.