Dzm. Wang et al., A NEW RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM IN THE FIRST INTRON OFTHE SPONTANEOUSLY HYPERTENSIVE RAT RENIN GENE, Clinical and experimental pharmacology and physiology, 21(4), 1994, pp. 339-341
1. Amplification of the entire first intron of the renin gene of spont
aneously hypertensive rat (SHR), Wistar-Kyoto (WKY) and Sprague-Dawley
rats (SD) followed by Bgl II digestion uncovered a new deletion (simi
lar to 50 bp) which exists upstream of the SHR renin gene first intron
tandem repeat element. 2. The SHR tandem repeat element was 600 bp sh
orter than that in the WKY while the WKY tandem repeat element was 280
bp shorter than that in the SD. 3. Since elements regulating gene exp
ression are known to exist in the first intron of other genes, this ne
w restriction fragment length polymorphism (RFLP) might play a role in
the reported overexpression of the SHR renin gene independent of chan
ges in the length of the tandem repeat element.