CHARACTERIZATION OF 2 MONOCLONAL-ANTIBODIES DIRECTED AGAINST SEROVAR-A HAEMOPHILUS-PARAGALLINARUM

Two monoclonal antibodies (MAbs) raised against a serovar A Haemophilu s paragallinarum were evaluated for their ability to react with 11 ref erence strains that represented all the recognized serovars and with 2 7 field isolates of Page serovar A collected from around the world. Th e MAbs were used in a hemagglutination-inhibition assay. Both MAbs rec ognized type strains of Page serovar A and Kume serovars A-1 and A-2 b ut not the type strains of Kume serovars A-3 and A-4. Neither MAb reco gnized the type strains of Page serovars B and C or Kume serovars B-1, C-1, C-2, C-3, or C-4. When evaluated with the 27 Page serovar A fiel d isolates, both MAbs recognized only 10 isolates. All of the recogniz ed isolates belonged to Kume serovars A-1 (nine isolates) or A-2 (one isolate). All of the field isolates that were not recognized by one or the other of the MAbs either were Kume serovar A-4 (seven isolates) o r could not be placed in an existing Kume A serovar (10 isolates). The results indicate that the epitope recognized by these MAbs is present only in strains of H. paragallinarum that belong to Kume serovars A-1 and A-2.