Ks. Alva et al., MECHANISTIC STUDY OF ENZYME-CATALYZED POLYMERIZATION OF 8-HYDROXYQUINOLINE-5-SULFONATE USING NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY, Macromolecular rapid communications, 18(2), 1997, pp. 133-137
Horseradish peroxidase catalyzed polymerization of 8-hydroxyquinoline-
5-sulfonate (HQS) has been studied by in-situ NMR spectroscopy. The 2,
4 and 7 positions of the HQS are involved in oxidative free radical c
oupling with other HQS molecules with the order of reactivity being 7
greater than or equal to 2 greater than or equal to 4. A mechanism for
the oxidative free radical coupling and structure of the resulting po
lymer is proposed, which is supported by C-13 NMR spectroscopy.