NEUROPEPTIDE-Y INHIBITS VASOACTIVE INTESTINAL PEPTIDE-STIMULATED ADENYLYL-CYCLASE IN RAT VENTRAL PROSTATE

Authors
SOLANO RM CARMENA MJ GUIJARRO LG PRIETO JC
Citation
Rm. Solano et al., NEUROPEPTIDE-Y INHIBITS VASOACTIVE INTESTINAL PEPTIDE-STIMULATED ADENYLYL-CYCLASE IN RAT VENTRAL PROSTATE, Neuropeptides, 27(1), 1994, pp. 31-37
Citations number
30
Categorie Soggetti
Neurosciences,"Endocrynology & Metabolism
Journal title
Neuropeptides → ACNP
ISSN journal
01434179
Volume
27
Issue
1
Year of publication
1994
Pages
31 - 37
Database
ISI
SICI code
0143-4179(1994)27:1<31:NIVIPA>2.0.ZU;2-6
Abstract
Neuropeptide Y (NPY), a peptide present in the prostate gland, was fou nd to inhibit vasoactive intestinal peptide (VIP)-stimulated cyclic AM P accumulation in isolated rat prostatic epithelial cells as well as V IP-stimulated adenylyl cyclase activity in rat prostatic membranes. Th e inhibitory effect of NPY was selective for the VIP receptor/effector system since it was also observed when using pituitary adenylyl cycla se activating peptide (PACAP-27) which presumably recognizes VIP recep tors in this gland, but not when using unrelated substances such as is oproterenol or forskolin. NPY did not modify either the general lipid membrane microviscosity or the VIP-receptor binding. The inhibitory ef fect of VIP was blocked by pretreatment of the prostatic membranes wit h pertussis toxin. These results suggest the presence of NPY receptors in rat ventral prostate coupled in an inhibitory manner to adenylyl c yclase through a guanine nucleotide regulatory G(i) protein.