PERFORMANCE OF SILICA-BONDED QUINOLINOL AS A SELECTIVE STATIONARY-PHASE FOR PACKED CAPILLARY SUPERCRITICAL-FLUID CHROMATOGRAPHY

Selective stationary phases are of crucial importance in packed column supercritical fluid chromatography (SFC), and a potentially powerful selective stationary phase, silica-bonded 8-quinolinol, has been evalu ated. Its performance was compared with that of an aminopropyl modifie d silica. The 8-quinolinol columns could be used at temperatures up to 150-degrees-C, while the aminopropyl columns were degraded above 90-d egrees-C. The general performance of the quinolinol column was very go od when utilizing a mobile phase containing a polar modifier. Reduced plate heights of 4.8 to 3.8 and symmetrical peaks were obtained. Furth er, the quinolinol phase showed excellent selectivities under supercri tical conditions. This was demonstrated by the separation of fatty aci d methylesters and azaarenes. In addition, changes in selectivity coul d readily be made by loading with metal ions.