EVIDENCE FOR THE GLYCOSYLATION OF PORCINE SERUM TRANSFERRIN AT A SINGLE-SITE LOCATED WITHIN THE C-TERMINAL LOBE

In this study we report the number and location of the glycans on PST. Urea PAGE and SDS-PAGE have been used to follow the enzymatic removal of sialic acids and of glycans from PST and the masses of native and deglycosylated PST have been determined by electrospray mass spectrome try. The results are consistent with the presence of a single biantenn ary glycan chain. As amino acid sequence analysis demonstrated the abs ence of a glycosylated asparagine at position 25, the glycosylation si te is restricted to Asp-497.