ENZYMATIC PEPTIDE-SYNTHESIS WITH FATTY ACID-MODIFIED CHYMOTRYPSIN ANDTRYPSIN IN AQUEOUS-ORGANIC MEDIA

Authors
KAWASAKI Y TAKAHASHI Y NAKATANI M MURAKAMI M DOSAKO S OKAI H
Citation
Y. Kawasaki et al., ENZYMATIC PEPTIDE-SYNTHESIS WITH FATTY ACID-MODIFIED CHYMOTRYPSIN ANDTRYPSIN IN AQUEOUS-ORGANIC MEDIA, Bioscience, biotechnology, and biochemistry, 58(6), 1994, pp. 1162-1163
Citations number
12
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
58
Issue
6
Year of publication
1994
Pages
1162 - 1163
Database
ISI
SICI code
0916-8451(1994)58:6<1162:EPWFAC>2.0.ZU;2-O
Abstract
Equilibrium controlled enzymatic peptide syntheses in aqueous-organic media were done with chymotrypsin and trypsin modified with varied fat ty acid. Palmitoyl-modified chymotrypsin and trypsin synthesised the p eptide at 1.7 and 2.4 times higher yield than those unmodified. Since an alteration of the substrate specificity was not observed, the chemi cal modification did not affect the active site of the enzymes.