DIFFERENTIATION OF AUJESZKYS-DISEASE VIRUS-STRAINS ISOLATED IN POLANDUSING DNA BIOTINYLATED PROBES

The aim of this study was to compare 17 different Aujeszky's disease v irus (ADV) isolates from clinical outbreaks of AD by using DNA biotiny lated probes. All isolates were collected in Poland between 1984 and 1 991. The restriction fragment pattern (RFP) analysis done by hybridiza tion to NIA-3 DNA biotinylated probe indicated that all Polish ADV fie ld strains can be classified as type I of Suid herpesvoirus 1. Hybridi zation with BamHI fragment 7 and gI gene biotinylated probes revealed an unusual heterogeneity of BamHI fragment 7 in almost 50% of strains isolated in Poland. The nature of the molecular changes in this fragme nt will be discussed.