GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR IMPROVES SURVIVAL IN2 MODELS OF GUT-DERIVED SEPSIS BY IMPROVING GUT BARRIER FUNCTION AND MODULATING BACTERIAL CLEARANCE
R. Gennari et al., GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR IMPROVES SURVIVAL IN2 MODELS OF GUT-DERIVED SEPSIS BY IMPROVING GUT BARRIER FUNCTION AND MODULATING BACTERIAL CLEARANCE, Annals of surgery, 220(1), 1994, pp. 68-76
Objective The effect of recombinant murine granulocyte macrophage colo
ny-stimulating factor (rmGM-CSF) on survival and host defense was stud
ied using two clinically relevant models of infection that included tr
ansfusion-induced immunosuppression. Summary Background Data Granulocy
te macrophage colony-stimulating factor improves resistance in several
models of infection, but its role in transfusion-induced immunosuppre
ssion and bacterial translocation (gut-derived sepsis) has not been de
fined. Methods Balb/c mice were treated with 100 ng of rmGM-CSF or pla
cebo for 6 days in a model of transfusion, burn, and gavage, of cecal
ligation and puncture (CLP). Translocation was studied in the first mo
del. Results Survival alter transfusion, burn, and gavage was 90% in r
mGM-CSF-treated animals versus 35% in the control group (p < 0.001). A
fter CLP, survival was 75% in the rmGM-CSF group versus 30% in the con
trol group (p = 0.01). Less translocation and better killing of bacter
ia was observed in the tissues in animals treated with rmGM-CSF. Concl
usion The ability of rmGM-CSF to improve gut barrier function and enha
nce killing of translocated organisms after burn injury-induced gut or
igin sepsis was associated with improved outcome. Granulocyte macropha
ge colony-stimulating factor also improved survival after CLP.