DERIVATIVES OF A NOVEL CYCLOPEPTOLIDE .1. SYNTHESIS, ANTIFUNGAL ACTIVITY, AND STRUCTURE-ACTIVITY-RELATIONSHIPS

The synthesis of a series of derivatives of the novel antifungal cyclo peptolide 1, which consists of nine S-amino acids and R-lactic acid, i s described. Besides functional group variation of MeAsp(4) (esters 2a -d, amides 3a-d, alcohol 4, and its derivatives) and Tyr(Me)(9) (demet hyl derivative 8, ethers 12a-f, 13, and oxidative degradation of the p henyl group to 14), opening of the lactone by LiOH in THF/H2O allowed manipulation of the hydroxy group of R-Hypr(10) in the resulting acycl ic peptide 15. Recyclization of 15 under Mitsunobu conditions followed by deprotection led to the S-Hypr(10) analogue 17 of 1. Cyclic decape ptides 33 and 34 as well as cyclic undecapeptides 35 and 36 were obtai ned via the corresponding modified linear peptides 23, 24, 27, and 28 by cyclization. Methylation of all secondary amide groups by CH3I and KH/18crown6 gave the permethylated compound 37. Two of the derivatives (17 and 34) showed superior activities against yeasts in vitro at pH 6.5 as compared to 1, but not at a lower pH (4.5).