MEMBRANE IONIC CURRENT PHOTOMODIFICATION BY ROSE-BENGAL AND MENADIONE- ROLE OF SINGLET OXYGEN

Photosensitized modification of ionic leak current and potassium curre nt was studied in frog cardiac atrial cells using whole cell patch cla mp techniques. Rose bengal (RB) and menadione (MQ) were used as photos ensitizers. Separate photophysical studies of the photosensitizers in deuterium oxide solution demonstrated that MQ did not produce singlet oxygen as evidenced by the lack of luminescence at 1270 nm, whereas RB was an efficient singlet oxygen generator. Both photosensitizers sens itized block of potassium current in atrial cells, and both sensitized an increase of ionic leak current. However, when photosensitizer conc entrations and illumination intensities were adjusted to match the rat e of block of potassium current by the two photosensitizers, there wer e dramatic differences in leak current increase, both quantitatively a nd qualitatively. Menadione sensitized a much slower increase in leak current than did RB. Further, the leak current sensitized by MQ had a more positive reversal potential than that sensitized by RB, suggestin g a less potassium-selective leak current pathway. The results suggest that, while the effects of singlet oxygen and non-singlet oxygen modi fication of cell membranes may be similar, there may also be significa nt differences in the resulting membrane permeabilities. The results a lso demonstrate that MQ and RB may be useful agents to study the role of singlet oxygen versus non-singlet oxygen modification of biological systems.