SITE SELECTIVITY IN THE GLYCATION OF ALPHA-A-CRYSTALLIN AND ALPHA-B-CRYSTALLIN BY GLUCOSE

Authors
ABRAHAM EC CHERIAN M SMITH JB
Citation
Ec. Abraham et al., SITE SELECTIVITY IN THE GLYCATION OF ALPHA-A-CRYSTALLIN AND ALPHA-B-CRYSTALLIN BY GLUCOSE, Biochemical and biophysical research communications, 201(3), 1994, pp. 1451-1456
Citations number
18
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
201
Issue
3
Year of publication
1994
Pages
1451 - 1456
Database
ISI
SICI code
0006-291X(1994)201:3<1451:SSITGO>2.0.ZU;2-4
Abstract
We determined the site selectivity of glycation by glucose (glucosylat ion) in alpha A- and alpha B-crystallins using two independent approac hes. HPLC purified C-14-glucose labeled chymotryptic peptides and affi nity chromatography/HPLC purified fully glycated peptides were identif ied by FAB-MS. Lys 11 and 78 of alpha A-crystallin and Lys 90 and/or 9 2 of alpha B-crystallin were the fast reacting sites of glucosylation. (C) 1994 Academic Press, Inc.